Custom DNA Vector Based siRNA Services
siRNA (RNA interference) becomes a power tool for
studying gene functions or target validation.
Using DNA vector based siRNA
approach, a small DNA sequence encoding a short hairpin RNA targeting the gene
of interest is cloned into our PsiRNA vector. The insert-containing vector can
be transfected into the cells, and subsequently expresses the short hairpin RNA.
DICER processes the short hairpin into ~22 nt double-stranded duplexes, which
hybridizes to the target RNA and directs target degradation by enzymatic
cleavage. Vicgene’s custom siRNA
Customers provide us the sequence of the designed siRNA.
Synthesis of the siRNA insert oligonucleotides
Cloning of the annealed oligonucleotides in PsiRNA vector
Sequencing of the siRNA insert
The final plasmid will be delivered with the sequence data
The price is $425/target
The constructs also contain
special DNA sequences to enhance the integration of the entire constructs into
host cell chromosomes in high frequency. To quantitatively assay gene
silece efficiency, Vicgene also provides mRNA detection kit to the
corresponding siRNA products